p2207 pGEM p53
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||10853||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2870
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1200
Entrez GeneTP53 (a.k.a. BCC7, LFS1, P53, TRP53)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
See author's map. Good for in vitro expression.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p2207 pGEM p53 was a gift from Peter Howley (Addgene plasmid # 10853)
For your References section:Association of human papillomavirus types 16 and 18 E6 proteins with p53. Werness BA, Levine AJ, Howley PM. Science. 1990 Apr 6. 248(4951):76-9. 10.1126/science.2157286 PubMed 2157286
Map uploaded by the depositor.