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pScI_dCas9-CDA_J23119-sgRNA
(Plasmid #108550)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 108550 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSC101
  • Backbone size w/o insert (bp) 9263
  • Total vector size (bp) 10639
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol low (12.5 ug/ml)
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Top10
  • Growth instructions
    Allow cells to recover for 2 hr in SOC medium. Transformed cells grow slow and colony formation takes 2 days. Do not pick larger colonies as escape cells may appear.
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    dCas9-PmCDA1
  • Species
    Synthetic; Streptococcus pyogenes
  • Insert Size (bp)
    7252
  • Mutation
    D10A and H840A for SpCas9
  • GenBank ID
    OYN72315.1 ABO15149.1
  • Promoter lambda OR
  • Tag / Fusion Protein
    • Flag

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Streptococcus pyogenes sgRNA
  • Species
    Synthetic
  • Insert Size (bp)
    138
  • Promoter J23119

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ggccctgcaaccattatc
  • 3′ sequencing primer gcgtggatccggcttac
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pScI_dCas9-CDA_J23119-sgRNA was a gift from Akihiko Kondo (Addgene plasmid # 108550 ; http://n2t.net/addgene:108550 ; RRID:Addgene_108550)
  • For your References section:

    Deaminase-mediated multiplex genome editing in Escherichia coli. Banno S, Nishida K, Arazoe T, Mitsunobu H, Kondo A. Nat Microbiol. 2018 Feb 5. pii: 10.1038/s41564-017-0102-6. doi: 10.1038/s41564-017-0102-6. 10.1038/s41564-017-0102-6 [pii] PubMed 29403014