pScI_dCas9-CDA-UL
(Plasmid #108551)

• Purpose: Bacterial Target-AID UGI-LVA vector
• Depositing Lab: Akihiko Kondo
• Publication: Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/s41564-017-0102-6. doi: 10.1038/s41564-017-0102-6.

Backbone

• Vector backbone: pSC101
• Backbone size w/o insert (bp): 9263
• Total vector size (bp): 10930
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 12.5 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Top10
• Growth instructions: Allow cells to recover for 2 hr in SOC medium. Transformed cells grow slow and colony formation takes 2 days. Do not pick larger colonies as escape cells may appear.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: dCas9-PmCDA1-UGI-LVA
• Species: Synthetic; Streptococcus pyogenes
• Insert Size (bp): 7252
• Mutation: D10A and H840A for SpCas9
• GenBank ID: OYN72315.1 ABO15149.1
• Promoter: lambda OR
• Tag / Fusion Protein:
  • Flag

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: atccggcttactaaaagcc
• 3′ sequencing primer: M13 F

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry