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ATG-1929
(Plasmid #108712)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 108712 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pF4Ag
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3820
  • Total vector size (bp) 5449
  • Vector type
    Mammalian Expression, Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CBR2opt
  • Species
    Pyrophorus plagiophthalamus
  • Insert Size (bp)
    1629
  • Mutation
    Promega used directed evolution to create CBR (Click Beetle Red). CBR2 contains 2 additional mutations (R344S+G351R) This gene is also codon optimized for mouse expression
  • Promoter T7, CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sgf1 (not destroyed)
  • 3′ cloning site Pme1 (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Promega
  • Articles Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ATG-1929 was a gift from Keith Wood (Addgene plasmid # 108712 ; http://n2t.net/addgene:108712 ; RRID:Addgene_108712)
  • For your References section:

    Click beetle luciferase mutant and near infrared naphthyl-luciferins for improved bioluminescence imaging. Hall MP, Woodroofe CC, Wood MG, Que I, Van't Root M, Ridwan Y, Shi C, Kirkland TA, Encell LP, Wood KV, Lowik C, Mezzanotte L. Nat Commun. 2018 Jan 9;9(1):132. doi: 10.1038/s41467-017-02542-9. 10.1038/s41467-017-02542-9 [pii] PubMed 29317625