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Plasmid 10881: pCCL cPPT PGK EGFP WPRE LTR H1 shSOD1mismatch
  • H1-shSOD1mismatch

  • SOD1

  • 394

  • H. sapiens (human)

  • SOD1 (ALS, ALS1, HEL-S-44, IPOA, SOD, hSod1, homodimer)

  • pCCL-cPPT-PGK-EGFP-WPRE
    (Search Vector Database)

  • Mammalian Expression, Lentiviral, RNAi, Cre/Lox

  • 7751

  • ClaI

  • Yes

  • ClaI

  • Yes

  • H1 specific primer List of Sequencing Primers

  • Ampicillin

  • Stbl3

  • 37

  • Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C

  • High Copy

  • View sequences (1)
  • Patrick Aebischer

  • UBMTA
    Ancillary Agreement for Plasmids Containing FP Materials

Comments: 

shSOD1 mismatch sense oligonucleotide: 5'-GATCCCCAAG GATTAATAGA GGCATGTTCA AGAGACATGC CTCTATTAAT CCTTTTTTTG GAAA-3'.

Please visit LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Lentiviral-mediated silencing of SOD1 through RNA interference retards disease onset and progression in a mouse model of ALS. Raoul et al (Nat Med. 2005 Apr . 11(4):423-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 10881" in your Materials and Methods section.