Addgene - pCCL cPPT PGK EGFP WPRE LTR H1 shSOD1mismatch Plasmid Data

Plasmid 10881: pCCL cPPT PGK EGFP WPRE LTR H1 shSOD1mismatch

Gene/insert name:
H1-shSOD1mismatch
Alt name:
SOD1
Insert size:
394
Species:
H. sapiens (human)
Entrez Gene:
SOD1 (ALS, ALS1, IPOA, SOD, hSod1, homodimer)
Vector backbone:
pCCL-cPPT-PGK-EGFP-WPRE
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral, RNAi, Cre/Lox
Backbone size w/o insert (bp):
7751
Cloning site 5':
ClaI
Site destroyed during cloning:
Yes
Cloning site 3':
ClaI
Site destroyed during cloning:
Yes
5' sequencing primer:
H1 specific primer List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Patrick Aebischer
Terms and Licenses:
MTA

Comments:

shSOD1 mismatch sense oligonucleotide: 5'-GATCCCCAAG GATTAATAGA GGCATGTTCA AGAGACATGC CTCTATTAAT CCTTTTTTTG GAAA-3'.

Please visit LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Lentiviral-mediated silencing of SOD1 through RNA interference retards disease onset and progression in a mouse model of ALS. Raoul et al (Nat Med. 2005 Apr . 11(4):423-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 10881" in your Materials and Methods section.