pCCL cPPT PGK EGFP WPRE LTR H1 shSOD1mismatch
- Backbone size w/o insert (bp) 7751
Vector typeMammalian Expression, Lentiviral, RNAi, Cre/Lox
Growth in Bacteria
Growth instructionsUse Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
Copy numberHigh Copy
Full plasmid sequence is available only if provided by the depositing laboratory.
SpeciesH. sapiens (human)
Insert Size (bp)394
Entrez GeneSOD1 (a.k.a. ALS, ALS1, HEL-S-44, IPOA, SOD, hSod1, homodimer)
- Cloning method Restriction Enzyme
- 5′ cloning site ClaI (destroyed during cloning)
- 3′ cloning site ClaI (destroyed during cloning)
- 5′ sequencing primer H1 specific primer (Common Sequencing Primers)
Terms and Licenses
shSOD1 mismatch sense oligonucleotide: 5'-GATCCCCAAG GATTAATAGA GGCATGTTCA AGAGACATGC CTCTATTAAT CCTTTTTTTG GAAA-3'.
Please visit LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCCL cPPT PGK EGFP WPRE LTR H1 shSOD1mismatch was a gift from Patrick Aebischer (Addgene plasmid # 10881)
For your References section:Lentiviral-mediated silencing of SOD1 through RNA interference retards disease onset and progression in a mouse model of ALS. Raoul C, Abbas-Terki T, Bensadoun JC, Guillot S, Haase G, Szulc J, Henderson CE, Aebischer P. Nat Med. 2005 Apr . 11(4):423-8. 10.1038/nm1207 PubMed 15768028
Map generated by Addgene from plasmid data.