Addgene - pWZL-blast Myr-FLAG-p110alpha Plasmid Data

Plasmid 10889: pWZL-blast Myr-FLAG-p110alpha

Gene/insert name:
p110alpha
Alt name:
PI3K p110
Alt name:
PIK3CA
Insert size:
3600
Species:
B. taurus (bovine)
GenBank ID:
NM_174574
Entrez Gene:
PIK3CA (BOS_1038)
Fusion protein or tag:
Myr
Terminal:
N terminal on insert
Fusion protein or tag:
Flag
Terminal:
N terminal on insert
Vector backbone:
pWZL Blast
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
5100
Cloning site 5':
EagI
Site destroyed during cloning:
Unknown
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
pBABE 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Blasticidin
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
William Hahn
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Human mammary epithelial cell transformation through the activation of phosphatidylinositol 3-kinase. Zhao et al (Cancer Cell. 2003 May . 3(5):483-95. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 10889" in your Materials and Methods section.