Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at help@addgene.org. Learn more

pWZL-blast Myr-FLAG-p110alpha
(Plasmid #10889)

Add to Cart
Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pWZL Blast
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    p110alpha
  • Alt name
    PI3K p110
  • Alt name
    PIK3CA
  • Species
    B. taurus (bovine)
  • Insert Size (bp)
    3600
  • GenBank ID
    NM_174574
  • Entrez Gene
    PIK3CA (a.k.a. BOS_1038)
  • Tags / Fusion Proteins
    • Myr (N terminal on insert)
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EagI (unknown if destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pBABE 5'
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWZL-blast Myr-FLAG-p110alpha was a gift from William Hahn (Addgene plasmid # 10889)
  • For your References section:

    Human mammary epithelial cell transformation through the activation of phosphatidylinositol 3-kinase. Zhao JJ, Gjoerup OV, Subramanian RR, Cheng Y, Chen W, Roberts TM, Hahn WC. Cancer Cell. 2003 May . 3(5):483-95. 10.1016/S1535-6108(03)00088-6 PubMed 12781366