Addgene - 7859 pRS305-pUG72 Plasmid Data

Plasmid 10897: 7859 pRS305-pUG72

Gene/insert name:
Ura3
Insert size:
1500
Species:
S. cerevisiae (budding yeast)
Entrez Gene:
URA3 (YEL021W)
Vector backbone:
pRS305
(Search Vector Database)
Vector type:
Yeast Expression, Cre/Lox
Backbone size w/o insert (bp):
5500
Cloning site 5':
SacII
Site destroyed during cloning:
No
Cloning site 3':
PstI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
LEU2, URA3
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Susan Lindquist
Terms and Licenses:
MTA

Comments:

Yeast integrating plasmid. URA3 flanked by loxP sites.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Hsp90 potentiates the rapid evolution of new traits: drug resistance in diverse fungi. Cowen et al (Science. 2005 Sep 30. 309(5744):2185-9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 10897" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

10895: 7852 pUG72-HSC82out