Addgene - GFP-siRNA control Plasmid Data

Plasmid 10900: GFP-siRNA control

Gene/insert name:
siGFP
Alt name:
GFP siRNA
Insert size:
60
Vector backbone:
pSP-108
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
9439
Cloning site 5':
BstBI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
TRC-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Puromycin
Person or lab that originally
cloned the gene/insert:
Empty pSP108 vector can be obtained from Dr. Eric Fearson, Univ of Michigan
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

Control vector for siTwist plasmids.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Twist, a master regulator of morphogenesis, plays an essential role in tumor metastasis. Yang et al (Cell 2004 Jun 25;117(7):927-39. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 10900" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

Print

This is commonly requested with

1784: Twist-siRNA3

8456: Twist-siRNA5

1783: pBABE-puro-mTwist

8457: Twist-siRNA7

10677: pBabe HcRed