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pBABE-puro TPR-Met
(Plasmid #10902)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pBABE-puro
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    TPR-Met
  • Alt name
    c-Met
  • Alt name
    TPR
  • Alt name
    MET
  • Species
    H. sapiens (human)
  • Mutation
    Constitutively active form of the c-Met receptor
  • Entrez Gene
    MET (a.k.a. AUTS9, HGFR, RCCP2, c-Met)
  • Entrez Gene
    TPR

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pBABE 5'
  • 3′ sequencing primer pBABE-3
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    G. Vande Woude
  • Terms and Licenses

Depositor Comments

For a complete description of the TPR-met fusion, see PubMed ID: 3821733

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE-puro TPR-Met was a gift from Bob Weinberg (Addgene plasmid # 10902)
  • For your References section:

    The melanocyte differentiation program predisposes to metastasis after neoplastic transformation. Gupta PB, Kuperwasser C, Brunet JP, Ramaswamy S, Kuo WL, Gray JW, Naber SP, Weinberg RA. Nat Genet. 2005 Oct . 37(10):1047-54. 10.1038/ng1634 PubMed 16142232