Addgene - pBABE-puro TPR-Met Plasmid Data

Plasmid 10902: pBABE-puro TPR-Met

Gene/insert name:
TPR-Met
Alt name:
c-Met
Alt name:
TPR
Alt name:
MET
Species:
H. sapiens (human)
Entrez Gene:
MET (AUTS9, HGFR, RCCP2, c-Met)
TPR ()
Mutation:
Constitutively active form of the c-Met receptor
Vector backbone:
pBABE-puro
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
5100
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
SalI
Site destroyed during cloning:
No
5' sequencing primer:
pBABE 5' List of Sequencing Primers
3' sequencing primer:
pBABE-3
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Person or lab that originally
cloned the gene/insert:
G. Vande Woude
Sequence: View sequences (2)
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

For a complete description of the TPR-met fusion, see PubMed ID: 3821733

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The melanocyte differentiation program predisposes to metastasis after neoplastic transformation. Gupta et al (Nat Genet. 2005 Oct . 37(10):1047-54. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 10902" in your Materials and Methods section.