pXR003: CasRx gRNA cloning backbone Citations (24)
Originally described in: Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors.Konermann S, Lotfy P, Brideau NJ, Oki J, Shokhirev MN, Hsu PD Cell. 2018 Mar 8. pii: S0092-8674(18)30207-1. doi: 10.1016/j.cell.2018.02.033. PubMed Journal
Articles Citing pXR003: CasRx gRNA cloning backbone
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| Structural Basis for the RNA-Guided Ribonuclease Activity of CRISPR-Cas13d. Zhang C, Konermann S, Brideau NJ, Lotfy P, Wu X, Novick SJ, Strutzenberg T, Griffin PR, Hsu PD, Lyumkis D. Cell. 2018 Sep 20;175(1):212-223.e17. doi: 10.1016/j.cell.2018.09.001. PubMed |
| Optimized RNA-targeting CRISPR/Cas13d technology outperforms shRNA in identifying functional circRNAs. Zhang Y, Nguyen TM, Zhang XO, Wang L, Phan T, Clohessy JG, Pandolfi PP. Genome Biol. 2021 Jan 21;22(1):41. doi: 10.1186/s13059-021-02263-9. PubMed |
| In vivo partial reprogramming of myofibers promotes muscle regeneration by remodeling the stem cell niche. Wang C, Rabadan Ros R, Martinez-Redondo P, Ma Z, Shi L, Xue Y, Guillen-Guillen I, Huang L, Hishida T, Liao HK, Nunez Delicado E, Rodriguez Esteban C, Guillen-Garcia P, Reddy P, Izpisua Belmonte JC. Nat Commun. 2021 May 25;12(1):3094. doi: 10.1038/s41467-021-23353-z. PubMed |
| CRISPRpas: programmable regulation of alternative polyadenylation by dCas9. Shin J, Ding Q, Wang L, Cui Y, Baljinnyam E, Guvenek A, Tian B. Nucleic Acids Res. 2021 Jul 9. pii: 6318496. doi: 10.1093/nar/gkab519. PubMed |
| Efficient Inhibition of HIV Using CRISPR/Cas13d Nuclease System. Nguyen H, Wilson H, Jayakumar S, Kulkarni V, Kulkarni S. Viruses. 2021 Sep 16;13(9). pii: v13091850. doi: 10.3390/v13091850. PubMed |
| LncRNA SOX1-OT V1 acts as a decoy of HDAC10 to promote SOX1-dependent hESC neuronal differentiation. Xi J, Xu Y, Guo Z, Li J, Wu Y, Sun Q, Wang Y, Chen M, Zhu S, Bian S, Kang J. EMBO Rep. 2022 Feb 3;23(2):e53015. doi: 10.15252/embr.202153015. Epub 2021 Dec 20. PubMed |
| Ribosome 18S m(6)A methyltransferase METTL5 promotes pancreatic cancer progression by modulating cMyc translation. Huang H, Li H, Pan R, Wang S, Khan AA, Zhao Y, Zhu H, Liu X. Int J Oncol. 2022 Jan;60(1). pii: 9. doi: 10.3892/ijo.2021.5299. Epub 2021 Dec 31. PubMed |
| Targeted gene silencing in the nervous system with CRISPR-Cas13. Powell JE, Lim CKW, Krishnan R, McCallister TX, Saporito-Magrina C, Zeballos MA, McPheron GD, Gaj T. Sci Adv. 2022 Jan 21;8(3):eabk2485. doi: 10.1126/sciadv.abk2485. Epub 2022 Jan 19. PubMed |
| CRISPR/CasRx Proof-of-Concept for RNA Degradation: A Future Tool against RNA Viruses? Perez-SanJose D, de la Fuente MA, Serna Perez J, Simarro M, Eiros Bouza JM, Sanz-Munoz I. Pharmaceuticals (Basel). 2021 Dec 27;15(1):32. doi: 10.3390/ph15010032. PubMed |
CRISPR/Cas13 effectors have differing extents of off-target effects that limit their utility in eukaryotic cells.
Ai Y, Liang D, Wilusz JE.
Nucleic Acids Res. 2022 Mar 4. pii: 6542487. doi: 10.1093/nar/gkac159.
PubMed
Associated Plasmids |
| ADARs act as potent regulators of circular transcriptome in cancer. Shen H, An O, Ren X, Song Y, Tang SJ, Ke XY, Han J, Tay DJT, Ng VHE, Molias FB, Pitcheshwar P, Leong KW, Tan KK, Yang H, Chen L. Nat Commun. 2022 Mar 21;13(1):1508. doi: 10.1038/s41467-022-29138-2. PubMed |
| Chromatin-associated orphan snoRNA regulates DNA damage-mediated differentiation via a non-canonical complex. Han C, Sun LY, Luo XQ, Pan Q, Sun YM, Zeng ZC, Chen TQ, Huang W, Fang K, Wang WT, Chen YQ. Cell Rep. 2022 Mar 29;38(13):110421. doi: 10.1016/j.celrep.2022.110421. PubMed |
| SAFA facilitates chromatin opening of immune genes through interacting with anti-viral host RNAs. Cao L, Luo Y, Guo X, Liu S, Li S, Li J, Zhang Z, Zhao Y, Zhang Q, Gao F, Ji X, Gao X, Li Y, You F. PLoS Pathog. 2022 Jun 3;18(6):e1010599. doi: 10.1371/journal.ppat.1010599. eCollection 2022 Jun. PubMed |
| Evaluating the Impact of gRNA SNPs in CasRx Activity for Reducing Viral RNA in HCoV-OC43. Mayes CM, Santarpia J. Cells. 2022 Jun 7;11(12). pii: cells11121859. doi: 10.3390/cells11121859. PubMed |
| Negative autoregulation mitigates collateral RNase activity of repeat-targeting CRISPR-Cas13d in mammalian cells. Kelley CP, Haerle MC, Wang ET. Cell Rep. 2022 Aug 16;40(7):111226. doi: 10.1016/j.celrep.2022.111226. PubMed |
| Optimization of specific RNA knockdown in mammalian cells with CRISPR-Cas13. Burris BJD, Molina Vargas AM, Park BJ, O'Connell MR. Methods. 2022 Oct;206:58-68. doi: 10.1016/j.ymeth.2022.08.007. Epub 2022 Aug 17. PubMed |
| A single N6-methyladenosine site regulates lncRNA HOTAIR function in breast cancer cells. Porman AM, Roberts JT, Duncan ED, Chrupcala ML, Levine AA, Kennedy MA, Williams MM, Richer JK, Johnson AM. PLoS Biol. 2022 Nov 28;20(11):e3001885. doi: 10.1371/journal.pbio.3001885. eCollection 2022 Nov. PubMed |
| Transcription of MERVL retrotransposons is required for preimplantation embryo development. Sakashita A, Kitano T, Ishizu H, Guo Y, Masuda H, Ariura M, Murano K, Siomi H. Nat Genet. 2023 Mar;55(3):484-495. doi: 10.1038/s41588-023-01324-y. Epub 2023 Mar 2. PubMed |
| Collateral activity of the CRISPR/RfxCas13d system in human cells. Shi P, Murphy MR, Aparicio AO, Kesner JS, Fang Z, Chen Z, Trehan A, Guo Y, Wu X. Commun Biol. 2023 Mar 28;6(1):334. doi: 10.1038/s42003-023-04708-2. PubMed |
| Multiplexed inhibition of immunosuppressive genes with Cas13d for on-demand combinatorial cancer immunotherapy. Zhang F, Guangchuan W, Chow R, He E, Majety M, Zhang Y, Chen S. bioRxiv. 2023 Apr 17:2023.03.14.532668. doi: 10.1101/2023.03.14.532668. Preprint. PubMed |
| AAV-CRISPR-Cas13 eliminates human enterovirus and prevents death of infected mice. Keng CT, Yogarajah T, Lee RCH, Muhammad IBH, Chia BS, Vasandani SR, Lim DS, Guo K, Wong YH, Mok CK, Chu JJH, Chew WL. EBioMedicine. 2023 Jul;93:104682. doi: 10.1016/j.ebiom.2023.104682. Epub 2023 Jun 28. PubMed |
| Complementary Alu sequences mediate enhancer-promoter selectivity. Liang L, Cao C, Ji L, Cai Z, Wang D, Ye R, Chen J, Yu X, Zhou J, Bai Z, Wang R, Yang X, Zhu P, Xue Y. Nature. 2023 Jul;619(7971):868-875. doi: 10.1038/s41586-023-06323-x. Epub 2023 Jul 12. PubMed |
| Noncoding function of super enhancer derived mRNA in modulating neighboring gene expression and TAD interaction. Xie B, Dean A. bioRxiv [Preprint]. 2023 Dec 5:2023.12.05.570115. doi: 10.1101/2023.12.05.570115. PubMed |
| CRISPR-based resistance to grapevine virus A. Spencer KP, Burger JT, Campa M. Front Plant Sci. 2023 Dec 4;14:1296251. doi: 10.3389/fpls.2023.1296251. eCollection 2023. PubMed |
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