Addgene - pcDNA3-E2F4 Plasmid Data

Plasmid 10914: pcDNA3-E2F4

Gene/insert name:
E2F4
Species:
H. sapiens (human)
GenBank ID:
U15641
Entrez Gene:
E2F4 (E2F-4)
Vector backbone:
pcDNA3
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5400
Cloning site 5':
NotI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
Sp6
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

See author's map.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: E2F-4 and E2F-5, two members of the E2F family, are expressed in the early phases of the cell cycle. Sardet et al (Proc Natl Acad Sci U S A. 1995 Mar 14. 92(6):2403-7. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 10914" in your Materials and Methods section.