Addgene - pSV2 neuNT Plasmid Data

Plasmid 10918: pSV2 neuNT

Gene/insert name:
neuNT
Alt name:
neu
Alt name:
p185
Insert size:
5000
Species:
R. norvegicus (rat)
Entrez Gene:
Erbb2 ()
Vector backbone:
pSV2 neo
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4400
Cloning site 5':
HindIII
Site destroyed during cloning:
No
Cloning site 3':
SalI
Site destroyed during cloning:
No
5' sequencing primer:
na List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

See author's map. The neu cDNA was inserted between the HindIII and SmaI sites of pSV2-neo, removing the neo gene. Has normal neu cDNA as in pSV2 neuN with transforming sequence (1899-2387) of pSV2 neuT. Expressed in NIH cells at higher levels than pSV2 neuT.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Multiple independent activations of the neu oncogene by a point mutation altering the transmembrane domain of p185. Bargmann et al (Cell. 1986 Jun 6. 45(5):649-57. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 10918" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

Print

This is commonly requested with

16259: HER2 CA (V659E)