Addgene - pEXL flag Smad3 Plasmid Data

Plasmid 10920: pEXL flag Smad3

Gene/insert name:
Smad3
Species:
H. sapiens (human)
Entrez Gene:
SMAD3 (HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3)
Fusion protein or tag:
flag
Terminal:
N terminal on insert
Vector backbone:
pEXL (derived from pEGFP-N1)
(Search Vector Database)
Backbone manufacturer:
EGFP-N1 is from Clontech
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4750
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-Forward List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (1)
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

This is a modified version of pEGFP-N1 where the GFP is replaced with flag-Smad3.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Transforming growth factor beta-induced phosphorylation of Smad3 is required for growth inhibition and transcriptional induction in epithelial cells. Liu et al (Proc Natl Acad Sci U S A. 1997 Sep 30. 94(20):10669-74. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 10920" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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