pFLAG/HA-DGCR8
(Plasmid #10921)

• Depositing Lab: Thomas Tuschl
• Publication: Landthaler et al Curr Biol. 2004 Dec 14. 14(23):2162-7.

Backbone

• Vector backbone: N/A
• Backbone size w/o insert (bp): 5283
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DGCR8
• Species: H. sapiens (human)
• Insert Size (bp): 2439
• GenBank ID: NM_022720
• Entrez Gene: DGCR8 (a.k.a. C22orf12, DGCRK6, Gy1, pasha)
• Tags / Fusion Proteins:
  • FLAG (N terminal on insert)
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV-F

Resource Information

• Articles Citing this Plasmid:
  • 10 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry