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pFLAG/HA-DGCR8
(Plasmid #10921)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 10921 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    N/A
  • Backbone size w/o insert (bp) 5283
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DGCR8
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2439
  • GenBank ID
    NM_022720
  • Entrez Gene
    DGCR8 (a.k.a. C22orf12, DGCRK6, Gy1, pasha)
  • Tags / Fusion Proteins
    • FLAG (N terminal on insert)
    • HA (N terminal on insert)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFLAG/HA-DGCR8 was a gift from Thomas Tuschl (Addgene plasmid # 10921)
  • For your References section:

    The human DiGeorge syndrome critical region gene 8 and Its D. melanogaster homolog are required for miRNA biogenesis. Landthaler M, Yalcin A, Tuschl T. Curr Biol. 2004 Dec 14. 14(23):2162-7. 10.1016/j.cub.2004.11.001 PubMed 15589161