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pT2K-LRL-mouse c-MYC-IRES-Luc2
(Plasmid #109228)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 109228 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pT2K-CAGGS
  • Backbone size w/o insert (bp) 8083
  • Vector type
    Mammalian Expression
  • Selectable markers
    luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    c-Myc
  • Alt name
    LoxP-DsRed2-LoxP (LRL)-Myc
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1400
  • Entrez Gene
    Myc (a.k.a. AU016757, Myc2, Niard, Nird, bHLHe39)
  • Promoter CAGGS
  • Tag / Fusion Protein
    • IRES-Luc2 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT2K-LRL-mouse c-MYC-IRES-Luc2 was a gift from Martine Roussel (Addgene plasmid # 109228 ; http://n2t.net/addgene:109228 ; RRID:Addgene_109228)
  • For your References section:

    Novel MYC-driven medulloblastoma models from multiple embryonic cerebellar cells. Kawauchi D, Ogg RJ, Liu L, Shih DJH, Finkelstein D, Murphy BL, Rehg JE, Korshunov A, Calabrese C, Zindy F, Phoenix T, Kawaguchi Y, Gronych J, Gilbertson RJ, Lichter P, Gajjar A, Kool M, Northcott PA, Pfister SM, Roussel MF. Oncogene. 2017 Sep 14;36(37):5231-5242. doi: 10.1038/onc.2017.110. Epub 2017 May 15. 10.1038/onc.2017.110 PubMed 28504719