pSUM-Kan-MCS2
(Plasmid
#109289)
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Purpose(Empty Backbone) Expresses high levels of protein in mycobacteria
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 109289 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepSUM36
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Backbone manufacturerfrom PMID 9218713
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
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MutationpSUM-kan-MCS1-gfp was modified to reorient the BamHI and HindII sites for directional cloning.
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pSUM-kan-MCS1-gfp was modified to flip the BamHI and HindII sites for directional cloning. The optimized translation initiation site (from pGreenTIR) was cloned at the 3' end of the kanamycin cassette.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSUM-Kan-MCS2 was a gift from Nicolai van Oers (Addgene plasmid # 109289 ; http://n2t.net/addgene:109289 ; RRID:Addgene_109289) -
For your References section:
Mycobacterial shuttle vectors designed for high-level protein expression in infected macrophages. Eitson JL, Medeiros JJ, Hoover AR, Srivastava S, Roybal KT, Ainsa JA, Hansen EJ, Gumbo T, van Oers NS. Appl Environ Microbiol. 2012 Oct;78(19):6829-37. doi: 10.1128/AEM.01674-12. Epub 2012 Jul 20. 10.1128/AEM.01674-12 PubMed 22820329
