PurposeArabinose inducible expression of the Vibrio Fischery Lux operon. Used to test burden response in E. coli
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||109383||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepSB1C3 (pMB1 origin of replication and CmR)
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namepARABAD-LUX operon
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSB1C3-Lux was a gift from Tom Ellis (Addgene plasmid # 109383 ; http://n2t.net/addgene:109383 ; RRID:Addgene_109383)
For your References section:Burden-driven feedback control of gene expression. Ceroni F, Boo A, Furini S, Gorochowski TE, Borkowski O, Ladak YN, Awan AR, Gilbert C, Stan GB, Ellis T. Nat Methods. 2018 Mar 26. pii: nmeth.4635. doi: 10.1038/nmeth.4635. 10.1038/nmeth.4635 PubMed 29578536