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pFLAG/HA-MOV10
(Plasmid #10976)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 10976 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    N/A
  • Backbone size w/o insert (bp) 5224
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MOV10
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3012
  • GenBank ID
    NP_066014
  • Entrez Gene
    MOV10 (a.k.a. fSAP113, gb110)
  • Tags / Fusion Proteins
    • FLAG (N terminal on insert)
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attR1 (not destroyed)
  • 3′ cloning site attR2 (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFLAG/HA-MOV10 was a gift from Thomas Tuschl (Addgene plasmid # 10976)
  • For your References section:

    Identification of Novel Argonaute-Associated Proteins. Meister G, Landthaler M, Peters L, Chen PY, Urlaub H, Luhrmann R, Tuschl T. Curr Biol. 2005 Nov 9. ():. 10.1016/j.cub.2005.10.048 PubMed 16289642