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pACEC3GH (Apr)
(Plasmid #110085)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 110085 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pACEC3GH
  • Vector type
    Bacterial Expression
  • Selectable markers
    Apramycin
  • Tag / Fusion Protein
    • 3C cleavage site - GFP - His10 Tag (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Apramycin, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • 3C cleavage site - GFP - His10 Tag (C terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pACEC3GH (Apr) was a gift from Markus Seeger (Addgene plasmid # 110085 ; http://n2t.net/addgene:110085 ; RRID:Addgene_110085)
  • For your References section:

    A uniform cloning platform for mycobacterial genetics and protein production. Arnold FM, Hohl M, Remm S, Koliwer-Brandl H, Adenau S, Chusri S, Sander P, Hilbi H, Seeger MA. Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.1038/s41598-018-27687-5. 10.1038/s41598-018-27687-5 PubMed 29934571