|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||110257||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
Alt nameras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
SpeciesH. sapiens (human)
Entrez GeneRAC1 (a.k.a. MIG5, MRD48, Rac-1, TC-25, p21-Rac1)
/ Fusion Protein
- StrepII-TEV (N terminal on insert)
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
Use BL21 (DE3) Star for expression. PDB structure is a complex of (KALRNA-c001 & RAC1A-c007). 5o33: https://www.thesgc.org/structures/5o33.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:RAC1 (RAC1A-c007) was a gift from Nicola Burgess-Brown (Addgene plasmid # 110257 ; http://n2t.net/addgene:110257 ; RRID:Addgene_110257)