Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pCDB364
(Plasmid #110275)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 110275 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCDB364
  • Backbone size (bp) 5919
  • Modifications to backbone
    started with pET29 and exchanged the new multiple cloning site
  • Vector type
    Bacterial Expression
  • Promoter T7
  • Tags / Fusion Proteins
    • 10-His (N terminal on backbone)
    • mature DsbC (i.e. no signal peptide) (N terminal on backbone)
    • TEV cleavage peptide (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer AGCAATGGCACACTTG
  • 3′ sequencing primer GCTAGTTATTGCTCAGCGG
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

built from pET29b

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDB364 was a gift from Christopher Bahl (Addgene plasmid # 110275 ; http://n2t.net/addgene:110275 ; RRID:Addgene_110275)
  • For your References section:

    Cytosolic expression, solution structures, and molecular dynamics simulation of genetically encodable disulfide-rich de novo designed peptides. Buchko GW, Pulavarti SVSRK, Ovchinnikov V, Shaw EA, Rettie SA, Myler PJ, Karplus M, Szyperski T, Baker D, Bahl CD. Protein Sci. 2018 Aug 26. doi: 10.1002/pro.3453. 10.1002/pro.3453 PubMed 30152054