mCRY1 HA tag
(Plasmid #110298)

• Purpose: full length wt mouse Cry1 HA tag in pcDNA3.1
• Depositing Lab: Joseph Takahashi
• Publication: Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi: 10.1016/j.cell.2013.01.055.

Backbone

• Vector backbone: pCDNA 3.1 HA tag
• Backbone manufacturer: genscript
• Total vector size (bp): 5450
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: wt mcry1 HA tag
• Alt name: mouse CRY1
• Species: M. musculus (mouse)
• Mutation: wildtype
• Entrez Gene: Cry1 (a.k.a. Phll1)
• Promoter: CMV
• Tags / Fusion Proteins:
  • HA tag (C terminal on insert)
  • HA tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: not sure (unknown if destroyed)
• 3′ cloning site: not sure (unknown if destroyed)
• 5′ sequencing primer: not sure
• 3′ sequencing primer: Not sure

Resource Information

• A portion of this plasmid was derived from a plasmid made by: not sure

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry