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pHIV-BRAFV600E-mOrange2
(Plasmid #110732)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 110732 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BRAFV600E IRES H2B-mOrange2
  • Species
    H. sapiens (human)
  • Mutation
    BRAF V600E
  • Entrez Gene
    BRAF (a.k.a. B-RAF1, B-raf, BRAF1, NS7, RAFB1)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHIV-BRAFV600E-mOrange2 was a gift from Robert Judson-Torres (Addgene plasmid # 110732 ; http://n2t.net/addgene:110732 ; RRID:Addgene_110732)
  • For your References section:

    Bi-allelic Loss of CDKN2A Initiates Melanoma Invasion via BRN2 Activation. Zeng H, Jorapur A, Shain AH, Lang UE, Torres R, Zhang Y, McNeal AS, Botton T, Lin J, Donne M, Bastian IN, Yu R, North JP, Pincus L, Ruben BS, Joseph NM, Yeh I, Bastian BC, Judson RL. Cancer Cell. 2018 Jul 9;34(1):56-68.e9. doi: 10.1016/j.ccell.2018.05.014. 10.1016/j.ccell.2018.05.014 PubMed 29990501