pCiPS-NIFV
(Plasmid #110807)

• Purpose: Non-Integrating Foamy Virus pol-expressing helper plasmid with mutations in the DDE catalyic core motif of the integrase sequence, remaining as unintegated episomes in transduced cells
• Depositing Lab: David Russell
• Publication: Deyle et al J Virol. 2010 Sep;84(18):9341-9. doi: 10.1128/JVI.00394-10. Epub 2010 Jun 30.

Backbone

• Vector backbone: pCMV beta
• Backbone manufacturer: unknown
• Backbone size w/o insert (bp): 7164
• Total vector size (bp): 7842
• Modifications to backbone: Site-directed mutagenesis (QuikChange; Stratagene, La Jolla, CA) was used to introduce point mutations at nucleotides 2636 and 2807 of the pol open reading frame (accession number U21247).
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Pol
• Species: human spumaretrovirus (foamy virus)
• Insert Size (bp): 4152
• Mutation: 2 mutations in the DDE catalyic core motif of the integrase sequence to prevent integration
• GenBank ID: Y07724.1 U21247.1
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: unknown
• 3′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • FV Vector Production Protocol
  • FV Vector Genome QPCR Protocol

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry