Addgene - pET-22b DT 51E/148K Plasmid Data

Plasmid 11081: pET-22b DT 51E/148K

Gene/insert name:
DT
Alt name:
diptheria toxin
Insert size:
1600
Species:
Corynebacterium diphtheriae
Fusion protein or tag:
His
Terminal:
C terminal on backbone
Mutation:
51E/148K, catalytically inactive
Vector backbone:
pET-22b
(Search Vector Database)
Backbone manufacturer:
EMD Biosciences
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
5500
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
Unknown
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (4)
Principal Investigator:
John Collier
Terms and Licenses:
MTA
EMD Millipore Limited Use Label License/Brookhaven pET Assurance Letter

Comments:

Note: the author's sequence is a wildtype DT sequence.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11081" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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