Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene

pDEST-HisMBP Citations (25)

Originally described in: Gateway vectors for the production of combinatorially-tagged His6-MBP fusion proteins in the cytoplasm and periplasm of Escherichia coli.
Nallamsetty S, Austin BP, Penrose KJ, Waugh DS Protein Sci. 2005 Dec . 14(12):2964-71.
PubMed Journal

Articles Citing pDEST-HisMBP

Articles
Triazole-linked inhibitors of inosine monophosphate dehydrogenase from human and Mycobacterium tuberculosis. Chen L, Wilson DJ, Xu Y, Aldrich CC, Felczak K, Sham YY, Pankiewicz KW. J Med Chem. 2010 Jun 24;53(12):4768-78. doi: 10.1021/jm100424m. PubMed
The pluripotency regulator Zic3 is a direct activator of the Nanog promoter in ESCs. Lim LS, Hong FH, Kunarso G, Stanton LW. Stem Cells. 2010 Nov;28(11):1961-9. doi: 10.1002/stem.527. PubMed
Inositol pentakisphosphate isomers bind PH domains with varying specificity and inhibit phosphoinositide interactions. Jackson SG, Al-Saigh S, Schultz C, Junop MS. BMC Struct Biol. 2011 Feb 10;11:11. doi: 10.1186/1472-6807-11-11. PubMed
Purification, crystallization and preliminary crystallographic analysis of human dihydrodipicolinate synthase-like protein (DHDPSL). Bunker RD, Loomes KM, Baker EN. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Jan 1;68(Pt 1):59-62. doi: 10.1107/S1744309111048068. Epub 2011 Dec 24. PubMed
NPR3 and NPR4 are receptors for the immune signal salicylic acid in plants. Fu ZQ, Yan S, Saleh A, Wang W, Ruble J, Oka N, Mohan R, Spoel SH, Tada Y, Zheng N, Dong X. Nature. 2012 May 16;486(7402):228-32. doi: 10.1038/nature11162. PubMed
A divalent ion is crucial in the structure and dominant-negative function of ID proteins, a class of helix-loop-helix transcription regulators. Wong MV, Jiang S, Palasingam P, Kolatkar PR. PLoS One. 2012;7(10):e48591. doi: 10.1371/journal.pone.0048591. Epub 2012 Oct 30. PubMed
Phosphatidylinositol 4-kinase and phosphatidylinositol 4-phosphate 5-kinase assays. Im YJ, Brglez I, Dieck C, Perera IY, Boss WF. Methods Mol Biol. 2013;1009:163-74. doi: 10.1007/978-1-62703-401-2_15. PubMed
Determination of specific antibody responses to the six species of ebola and marburg viruses by multiplexed protein microarrays. Kamata T, Natesan M, Warfield K, Aman MJ, Ulrich RG. Clin Vaccine Immunol. 2014 Dec;21(12):1605-12. doi: 10.1128/CVI.00484-14. Epub 2014 Sep 17. PubMed
Two-tiered histidine kinase pathway involved in heat shock and salt sensing in the general stress response of Sphingomonas melonis Fr1. Kaczmarczyk A, Hochstrasser R, Vorholt JA, Francez-Charlot A. J Bacteriol. 2015 Apr;197(8):1466-77. doi: 10.1128/JB.00019-15. Epub 2015 Feb 9. PubMed
The cytokinin response factors modulate root and shoot growth and promote leaf senescence in Arabidopsis. Raines T, Shanks C, Cheng CY, McPherson D, Argueso CT, Kim HJ, Franco-Zorrilla JM, Lopez-Vidriero I, Solano R, Vankova R, Schaller GE, Kieber JJ. Plant J. 2016 Jan;85(1):134-47. doi: 10.1111/tpj.13097. PubMed
Crystal structures and mutagenesis of PPP-family ser/thr protein phosphatases elucidate the selectivity of cantharidin and novel norcantharidin-based inhibitors of PP5C. Chattopadhyay D, Swingle MR, Salter EA, Wood E, D'Arcy B, Zivanov C, Abney K, Musiyenko A, Rusin SF, Kettenbach A, Yet L, Schroeder CE, Golden JE, Dunham WH, Gingras AC, Banerjee S, Forbes D, Wierzbicki A, Honkanen RE. Biochem Pharmacol. 2016 Jun 1;109:14-26. doi: 10.1016/j.bcp.2016.03.011. Epub 2016 Mar 23. PubMed
Expression and Purification of Recombinant Proteins in Escherichia coli with a His6 or Dual His6-MBP Tag. Raran-Kurussi S, Waugh DS. Methods Mol Biol. 2017;1607:1-15. doi: 10.1007/978-1-4939-7000-1_1. PubMed
A Mechanism Coupling Systemic Energy Sensing to Adipokine Secretion. Rajan A, Housden BE, Wirtz-Peitz F, Holderbaum L, Perrimon N. Dev Cell. 2017 Oct 9;43(1):83-98.e6. doi: 10.1016/j.devcel.2017.09.007. PubMed
Regulation of Androgen Receptor Activity by Transient Interactions of Its Transactivation Domain with General Transcription Regulators. De Mol E, Szulc E, Di Sanza C, Martinez-Cristobal P, Bertoncini CW, Fenwick RB, Frigole-Vivas M, Masin M, Hunter I, Buzon V, Brun-Heath I, Garcia J, De Fabritiis G, Estebanez-Perpina E, McEwan IJ, Nebreda AR, Salvatella X. Structure. 2018 Jan 2;26(1):145-152.e3. doi: 10.1016/j.str.2017.11.007. Epub 2017 Dec 7. PubMed
gamma-TuRC Heterogeneity Revealed by Analysis of Mozart1. Tovey CA, Tubman CE, Hamrud E, Zhu Z, Dyas AE, Butterfield AN, Fyfe A, Johnson E, Conduit PT. Curr Biol. 2018 Jul 23;28(14):2314-2323.e6. doi: 10.1016/j.cub.2018.05.044. Epub 2018 Jul 5. PubMed
A Smartphone-Based Rapid Telemonitoring System for Ebola and Marburg Disease Surveillance. Natesan M, Wu SW, Chen CI, Jensen SMR, Karlovac N, Dyas BK, Mudanyali O, Ulrich RG. ACS Sens. 2019 Jan 25;4(1):61-68. doi: 10.1021/acssensors.8b00842. Epub 2018 Dec 28. PubMed
Mutations of the AtYAK1 Kinase Suppress TOR Deficiency in Arabidopsis. Forzani C, Duarte GT, Van Leene J, Clement G, Huguet S, Paysant-Le-Roux C, Mercier R, De Jaeger G, Leprince AS, Meyer C. Cell Rep. 2019 Jun 18;27(12):3696-3708.e5. doi: 10.1016/j.celrep.2019.05.074. PubMed
Hsp70 and Hsp40 inhibit an inter-domain interaction necessary for transcriptional activity in the androgen receptor. Eftekharzadeh B, Banduseela VC, Chiesa G, Martinez-Cristobal P, Rauch JN, Nath SR, Schwarz DMC, Shao H, Marin-Argany M, Di Sanza C, Giorgetti E, Yu Z, Pierattelli R, Felli IC, Brun-Heath I, Garcia J, Nebreda AR, Gestwicki JE, Lieberman AP, Salvatella X. Nat Commun. 2019 Aug 8;10(1):3562. doi: 10.1038/s41467-019-11594-y. PubMed
Solanum lycopersicum (tomato) possesses mitochondrial and plastidial lipoyl synthases capable of increasing lipoylation levels when expressed in bacteria. Araya-Flores J, Miranda S, Covarrubias MP, Stange C, Handford M. Plant Physiol Biochem. 2020 Jun;151:264-270. doi: 10.1016/j.plaphy.2020.03.031. Epub 2020 Mar 25. PubMed
Autoinhibition of Cnn binding to gamma-TuRCs prevents ectopic microtubule nucleation and cell division defects. Tovey CA, Tsuji C, Egerton A, Bernard F, Guichet A, de la Roche M, Conduit PT. J Cell Biol. 2021 Aug 2;220(8). pii: 212197. doi: 10.1083/jcb.202010020. Epub 2021 May 27. PubMed
Intrinsically disordered protein biosensor tracks the physical-chemical effects of osmotic stress on cells. Cuevas-Velazquez CL, Vellosillo T, Guadalupe K, Schmidt HB, Yu F, Moses D, Brophy JAN, Cosio-Acosta D, Das A, Wang L, Jones AM, Covarrubias AA, Sukenik S, Dinneny JR. Nat Commun. 2021 Sep 14;12(1):5438. doi: 10.1038/s41467-021-25736-8. PubMed

Associated Plasmids

Male infertility-associated Ccdc108 regulates multiciliogenesis via the intraflagellar transport machinery. Zhao H, Sun J, Insinna C, Lu Q, Wang Z, Nagashima K, Stauffer J, Andresson T, Specht S, Perera S, Daar IO, Westlake CJ. EMBO Rep. 2022 Apr 5;23(4):e52775. doi: 10.15252/embr.202152775. Epub 2022 Feb 24. PubMed
Ancient origin and conserved gene function in terpene pheromone and defense evolution of stink bugs and hemipteran insects. Rebholz Z, Lancaster J, Larose H, Khrimian A, Luck K, Sparks ME, Gendreau KL, Shewade L, Kollner TG, Weber DC, Gundersen-Rindal DE, O'Maille P, Morozov AV, Tholl D. Insect Biochem Mol Biol. 2023 Jan;152:103879. doi: 10.1016/j.ibmb.2022.103879. Epub 2022 Dec 2. PubMed
The HAPSTR2 retrogene buffers stress signaling and resilience in mammals. Amici DR, Cingoz H, Alasady MJ, Alhayek S, Phoumyvong CM, Sahni N, Yi SS, Mendillo ML. Nat Commun. 2023 Jan 11;14(1):152. doi: 10.1038/s41467-022-35697-1. PubMed
A single amino acid change led to structural and functional differentiation of PvHd1 to control flowering in switchgrass. Choi S, Prabhakar PK, Chowdhury R, Pendergast Iv TH, Urbanowicz BR, Maranas C, Devos KM. J Exp Bot. 2023 Jul 4:erad255. doi: 10.1093/jxb/erad255. PubMed

If you have published an article using this material, please email us at [email protected] to have your article added to this page.