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PF3D7_1123500-COMP-blac-flag-his
(Plasmid #110987)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 110987 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTT3
  • Backbone manufacturer
    pTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute
  • Backbone size w/o insert (bp) 7661
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    conserved Plasmodium protein
  • Alt name
    PF3D7_1123500
  • Species
    Synthetic
  • Insert Size (bp)
    3597
  • Mutation
    All NXT/S sites mutated to NXA, where X is any amino acid except Proline
  • Entrez Gene
    PF3D7_1123500 (a.k.a. PF3D7_1123500, PF11_0246)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Exogenous signal peptide of mouse origin (N terminal on insert)
    • rat Cd4d3+4, beta-lactamase, triple FLAG tag, 6xHis tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Not1 (not destroyed)
  • 3′ cloning site Asc1 (not destroyed)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PF3D7_1123500-COMP-blac-flag-his was a gift from Gavin Wright (Addgene plasmid # 110987 ; http://n2t.net/addgene:110987 ; RRID:Addgene_110987)