pNIFV
(Plasmid #111085)

• Purpose: pNIFV is a vector plasmid with mutations in the cis-acting region, to prevent correction of the integrase by recombination when used with pCiPS-NIFV to produce Non-Integrating Foamy Virus vectors
• Depositing Lab: David Russell
• Publication: Deyle et al J Virol. 2010 Sep;84(18):9341-9. doi: 10.1128/JVI.00394-10. Epub 2010 Jun 30.

Backbone

• Vector backbone: deleted foamy vector pHSRV13
• Backbone manufacturer: R. M. Flugel 1991
• Backbone size w/o insert (bp): 3800
• Total vector size (bp): 6357
• Modifications to backbone: pNIFV was created by inserting a PstI-EcoRI restriction fragment from pCiPS-NIFV into the cis-acting pol region of pΔΦ. pΔΦ has deletions in the gag, pol, env, and bel1–3 accessory genes, as well as the LTR U3 region, but retains an essential 2.5-kb cis-acting region. In addition, a polylinker region to insert a transgene cassette, and stop codons were introduced into the remaining gag sequences to prevent expression of viral peptides and to eliminate dominant-negative effects of a Gag-Pol fusion protein.
• Vector type: deleted foamy virus
• Selectable markers: none; to be added in cassette

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: empty backbone/deleted foamy vector, same as pΔΦ with mutations in cis-acting pol region
• Species: human spumaretrovirus (foamy virus)
• Mutation: at nt 2334 A changed to T, at nt 2495 A changed to C
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PstI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: unknown
• 3′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • FV Vector Production Protocol
  • FV Vector Genome QPCR Protocol

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry