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pNIFV
(Plasmid #111085)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 111085 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    deleted foamy vector pHSRV13
  • Backbone manufacturer
    R. M. Flugel 1991
  • Backbone size w/o insert (bp) 3800
  • Total vector size (bp) 6357
  • Modifications to backbone
    pNIFV was created by inserting a PstI-EcoRI restriction fragment from pCiPS-NIFV into the cis-acting pol region of pΔΦ. pΔΦ has deletions in the gag, pol, env, and bel1–3 accessory genes, as well as the LTR U3 region, but retains an essential 2.5-kb cis-acting region. In addition, a polylinker region to insert a transgene cassette, and stop codons were introduced into the remaining gag sequences to prevent expression of viral peptides and to eliminate dominant-negative effects of a Gag-Pol fusion protein.
  • Vector type
    deleted foamy virus
  • Selectable markers
    none; to be added in cassette

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    empty backbone/deleted foamy vector, same as pΔΦ with mutations in cis-acting pol region
  • Species
    human spumaretrovirus (foamy virus)
  • Mutation
    at nt 2334 A changed to T, at nt 2495 A changed to C
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PstI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer unknown
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNIFV was a gift from David Russell (Addgene plasmid # 111085 ; http://n2t.net/addgene:111085 ; RRID:Addgene_111085)
  • For your References section:

    Nonintegrating foamy virus vectors. Deyle DR, Li Y, Olson EM, Russell DW. J Virol. 2010 Sep;84(18):9341-9. doi: 10.1128/JVI.00394-10. Epub 2010 Jun 30. 10.1128/JVI.00394-10 PubMed 20592072