LT3REVIN
(Plasmid #111175)

• Purpose: Tet-regulated miR-E (miR-30 variant)-based RNAi
• Depositing Lab: Johannes Zuber
• Publication: Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi: 10.1016/j.celrep.2013.11.020. Epub 2013 Dec 12.

Backbone

• Vector backbone: pRRL
• Vector type: Lentiviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: Please note that this plasmid is prone to recombination. Test 3-5 colonies to ensure the full plasmid is stored.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: miR-E (miR-30 variant)
• Alt name: T3G-DsRed-(miR-E)-PGK-Venus-IRES-Neo
• gRNA/shRNA sequence: Ren.713
• Species: H. sapiens (human)
• Mutation: WT
• Promoter: T3G

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)
• 5′ sequencing primer: DsRed1-C
• 3′ sequencing primer: MSCV-rev

Resource Information

• Supplemental Documents:
  • miRE 16_LT3REVIN_Ren713.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Tet Systems Limited Use Label License
• Industry Terms:
  • Not Available to Industry