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pbabe-c-mycT58A+HRasG12V
(Plasmid #11130)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11130 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pbabe
  • Backbone size w/o insert (bp) 4500
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    c-myc, HRas
  • Alt name
    c-myc
  • Alt name
    HRas
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Insert Size (bp)
    2300
  • Mutation
    c-myc T58A, A156T, and G438R; and H-Ras G12V
  • Entrez Gene
    HRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, KRAS, KRAS2, Ki-Ras, RASH1, RASK2, c-K-ras, c-Ki-ras, p21ras)
  • Entrez Gene
    Myc (a.k.a. AU016757, Myc2, Niard, Nird, bHLHe39)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI(c-myc), HindIII(H-Ras) (not destroyed)
  • 3′ cloning site EcoRI(c-myc), ClaI(H-Ras) (not destroyed)
  • 5′ sequencing primer pBABE 5'
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Contains mouse c-myc and human Hras. This plasmid was generated by cloning c-MycT58A cDNA with flanking BamHI-EcoRI restriction sites added by PCR into the same sites of the polylinker of pBABEpuro or pBABEblast and by excising the selectable marker from the HindIII/ClaI sites and ligating into the same site H-RasG12V cDNA in which HindIII/ClaI sites were again added by PCR.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pbabe-c-mycT58A+HRasG12V was a gift from Christopher Counter (Addgene plasmid # 11130 ; http://n2t.net/addgene:11130 ; RRID:Addgene_11130)
  • For your References section:

    A network of genetic events sufficient to convert normal human cells to a tumorigenic state. Kendall SD, Linardic CM, Adam SJ, Counter CM. Cancer Res. 2005 Nov 1. 65(21):9824-8. 10.1158/0008-5472.CAN-05-1543 PubMed 16267004