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PurposeExpress human GSDMD in E.coli
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 111559 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET SUMO
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Backbone manufacturerThermo Fisher Scientific
- Backbone size w/o insert (bp) 5600
- Total vector size (bp) 7098
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth TemperatureRoom Temperature
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameGasdermin D
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Alt nameGSDMD
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SpeciesH. sapiens (human)
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Insert Size (bp)1455
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Entrez GeneGSDMD (a.k.a. DF5L, DFNA5L, FKSG10, GSDMDC1)
- Promoter T7
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Tag
/ Fusion Protein
- His, SUMO (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer NA
- 3′ sequencing primer NA (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-SUMO-hGSDMD was a gift from Hongbo Luo (Addgene plasmid # 111559 ; http://n2t.net/addgene:111559 ; RRID:Addgene_111559) -
For your References section:
Gasdermin D Exerts Anti-inflammatory Effects by Promoting Neutrophil Death. Kambara H, Liu F, Zhang X, Liu P, Bajrami B, Teng Y, Zhao L, Zhou S, Yu H, Zhou W, Silberstein LE, Cheng T, Han M, Xu Y, Luo HR. Cell Rep. 2018 Mar 13;22(11):2924-2936. doi: 10.1016/j.celrep.2018.02.067. 10.1016/j.celrep.2018.02.067 PubMed 29539421
