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pEMS2274
(Plasmid #111900)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 111900 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEMS2131
  • Backbone manufacturer
    Elizabeth Simpson (Addgene plasmid # 111895)
  • Backbone size w/o insert (bp) 4999
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Ple344
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    801
  • Entrez Gene
    TUBB3 (a.k.a. CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, beta-4)
  • Promoter Ple344 (TUBB3 MiniPromoter)

Cloning Information

Resource Information

Depositor Comments

. Please see the annotated GenBank file and the additional image file for the specific location of features in this plasmid, including the MiniPromoter. Please note - an 11 bp insertion, which introduces a StuI site upstream of 5' ITR, was found during Addgene's Quality Control process.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEMS2274 was a gift from Elizabeth Simpson (Addgene plasmid # 111900 ; http://n2t.net/addgene:111900 ; RRID:Addgene_111900)
  • For your References section:

    New MiniPromoter Ple345 (NEFL) Drives Strong and Specific Expression in Retinal Ganglion Cells of Mouse and Primate Retina. Simpson E, Korecki AJ, Fornes O, McGill TJ, Cueva-Vargas JL, Agostinone J, Farkas RA, Hickmott JW, Lam SL, Mathelier A, Renner LM, Stoddard J, Zhou M, Di Polo A, Neuringer M, Wasserman WW. Hum Gene Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118. 10.1089/hum.2018.118 PubMed 30062914