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pCMV_ABEmax Citations (43)

Originally described in: Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction.
Koblan LW, Doman JL, Wilson C, Levy JM, Tay T, Newby GA, Maianti JP, Raguram A, Liu DR Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172. PubMed Journal

Articles Citing pCMV_ABEmax

Articles
Expanded targeting scope and enhanced base editing efficiency in rabbit using optimized xCas9(3.7). Liu Z, Chen M, Shan H, Chen S, Xu Y, Song Y, Zhang Q, Yuan H, Ouyang H, Li Z, Lai L. Cell Mol Life Sci. 2019 Apr 27. pii: 10.1007/s00018-019-03110-8. doi: 10.1007/s00018-019-03110-8. PubMed
CRISPR-Pass: Gene Rescue of Nonsense Mutations Using Adenine Base Editors. Lee C, Hyun Jo D, Hwang GH, Yu J, Kim JH, Park SE, Kim JS, Kim JH, Bae S. Mol Ther. 2019 May 24. pii: S1525-0016(19)30227-8. doi: 10.1016/j.ymthe.2019.05.013. PubMed
BEAT: A Python Program to Quantify Base Editing from Sanger Sequencing. Xu L, Liu Y, Han R. CRISPR J. 2019 Aug;2:223-229. doi: 10.1089/crispr.2019.0017. Epub 2019 Jul 18. PubMed
Highly efficient base editing with expanded targeting scope using SpCas9-NG in rabbits. Liu Z, Shan H, Chen S, Chen M, Song Y, Lai L, Li Z. FASEB J. 2020 Jan;34(1):588-596. doi: 10.1096/fj.201901587R. Epub 2019 Nov 26. PubMed
Computer simulations explain mutation-induced effects on the DNA editing by adenine base editors. Rallapalli KL, Komor AC, Paesani F. Sci Adv. 2020 Mar 4;6(10):eaaz2309. doi: 10.1126/sciadv.aaz2309. eCollection 2020 Mar. PubMed
BEON: A Functional Fluorescence Reporter for Quantification and Enrichment of Adenine Base-Editing Activity. Wang P, Xu L, Gao Y, Han R. Mol Ther. 2020 Jul 8;28(7):1696-1705. doi: 10.1016/j.ymthe.2020.04.009. Epub 2020 Apr 16. PubMed
Dual base editor catalyzes both cytosine and adenine base conversions in human cells. Zhang X, Zhu B, Chen L, Xie L, Yu W, Wang Y, Li L, Yin S, Yang L, Hu H, Han H, Li Y, Wang L, Chen G, Ma X, Geng H, Huang W, Pang X, Yang Z, Wu Y, Siwko S, Kurita R, Nakamura Y, Yang L, Liu M, Li D. Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0527-y. doi: 10.1038/s41587-020-0527-y. PubMed

Associated Plasmids
Base editors for simultaneous introduction of C-to-T and A-to-G mutations. Sakata RC, Ishiguro S, Mori H, Tanaka M, Tatsuno K, Ueda H, Yamamoto S, Seki M, Masuyama N, Nishida K, Nishimasu H, Arakawa K, Kondo A, Nureki O, Tomita M, Aburatani H, Yachie N. Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0509-0. doi: 10.1038/s41587-020-0509-0. PubMed

Associated Plasmids
AcrIIA5 Suppresses Base Editors and Reduces Their Off-Target Effects. Liang M, Sui T, Liu Z, Chen M, Liu H, Shan H, Lai L, Li Z. Cells. 2020 Jul 27;9(8). pii: cells9081786. doi: 10.3390/cells9081786. PubMed
CRISPR Start-Loss: A Novel and Practical Alternative for Gene Silencing through Base-Editing-Induced Start Codon Mutations. Chen S, Xie W, Liu Z, Shan H, Chen M, Song Y, Yu H, Lai L, Li Z. Mol Ther Nucleic Acids. 2020 Sep 4;21:1062-1073. doi: 10.1016/j.omtn.2020.07.037. Epub 2020 Jul 31. PubMed
Generation of a human embryonic stem cell line (WAe009-A-43) carrying a Long QT syndrome related mutation in KCNH2 gene. Wu X, Zhao Y, Wang X. Stem Cell Res. 2020 Oct;48:101976. doi: 10.1016/j.scr.2020.101976. Epub 2020 Sep 2. PubMed
Cytoprotection by a naturally occurring variant of ATP5G1 in Arctic ground squirrel neural progenitor cells. Singhal NS, Bai M, Lee EM, Luo S, Cook KR, Ma DK. Elife. 2020 Oct 14;9. pii: 55578. doi: 10.7554/eLife.55578. PubMed
Restoration of visual function in adult mice with an inherited retinal disease via adenine base editing. Suh S, Choi EH, Leinonen H, Foik AT, Newby GA, Yeh WH, Dong Z, Kiser PD, Lyon DC, Liu DR, Palczewski K. Nat Biomed Eng. 2021 Feb;5(2):169-178. doi: 10.1038/s41551-020-00632-6. Epub 2020 Oct 19. PubMed
Base Editing Mediated Generation of Point Mutations Into Human Pluripotent Stem Cells for Modeling Disease. Qi T, Wu F, Xie Y, Gao S, Li M, Pu J, Li D, Lan F, Wang Y. Front Cell Dev Biol. 2020 Sep 25;8:590581. doi: 10.3389/fcell.2020.590581. eCollection 2020. PubMed

Associated Plasmids
A Cas-embedding strategy for minimizing off-target effects of DNA base editors. Liu Y, Zhou C, Huang S, Dang L, Wei Y, He J, Zhou Y, Mao S, Tao W, Zhang Y, Yang H, Huang X, Chi T. Nat Commun. 2020 Nov 27;11(1):6073. doi: 10.1038/s41467-020-19690-0. PubMed
Delete_A Cas9-mediated adenosine transient reporter enables enrichment of ABE-targeted cells. Brookhouser N, Nguyen T, Tekel SJ, Standage-Beier K, Wang X, Brafman DA. BMC Biol. 2020 Dec 14;18(1):193. doi: 10.1186/s12915-020-00929-7. PubMed
Adenine Base Editor Ribonucleoproteins Delivered by Lentivirus-Like Particles Show High On-Target Base Editing and Undetectable RNA Off-Target Activities. Lyu P, Lu Z, Cho SI, Yadav M, Yoo KW, Atala A, Kim JS, Lu B. CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1089/crispr.2020.0095. PubMed

Associated Plasmids
Identification of pathogenic variants in cancer genes using base editing screens with editing efficiency correction. Huang C, Li G, Wu J, Liang J, Wang X. Genome Biol. 2021 Mar 10;22(1):80. doi: 10.1186/s13059-021-02305-2. PubMed
Structure-guided engineering of adenine base editor with minimized RNA off-targeting activity. Li J, Yu W, Huang S, Wu S, Li L, Zhou J, Cao Y, Huang X, Qiao Y. Nat Commun. 2021 Apr 16;12(1):2287. doi: 10.1038/s41467-021-22519-z. PubMed
Adenine base editing and prime editing of chemically derived hepatic progenitors rescue genetic liver disease. Kim Y, Hong SA, Yu J, Eom J, Jang K, Yoon S, Hong DH, Seo D, Lee SN, Woo JS, Jeong J, Bae S, Choi D. Cell Stem Cell. 2021 Sep 2;28(9):1614-1624.e5. doi: 10.1016/j.stem.2021.04.010. Epub 2021 May 4. PubMed
Correction of the pathogenic mutation in TGM1 gene by adenine base editing in mutant embryos. Dang L, Zhou X, Zhong X, Yu W, Huang S, Liu H, Chen Y, Zhang W, Yuan L, Li L, Huang X, Li G, Liu J, Tong G. Mol Ther. 2021 May 8. pii: S1525-0016(21)00259-8. doi: 10.1016/j.ymthe.2021.05.007. PubMed
In vivo gene editing via homology-independent targeted integration for adrenoleukodystrophy treatment. Hong SA, Seo JH, Wi S, Jung ES, Yu J, Hwang GH, Yu JH, Baek A, Park S, Bae S, Cho SR. Mol Ther. 2022 Jan 5;30(1):119-129. doi: 10.1016/j.ymthe.2021.05.022. Epub 2021 May 29. PubMed
In utero adenine base editing corrects multi-organ pathology in a lethal lysosomal storage disease. Bose SK, White BM, Kashyap MV, Dave A, De Bie FR, Li H, Singh K, Menon P, Wang T, Teerdhala S, Swaminathan V, Hartman HA, Jayachandran S, Chandrasekaran P, Musunuru K, Jain R, Frank DB, Zoltick P, Peranteau WH. Nat Commun. 2021 Jul 13;12(1):4291. doi: 10.1038/s41467-021-24443-8. PubMed
High-purity production and precise editing of DNA base editing ribonucleoproteins. Jang HK, Jo DH, Lee SN, Cho CS, Jeong YK, Jung Y, Yu J, Kim JH, Woo JS, Bae S. Sci Adv. 2021 Aug 27;7(35). pii: 7/35/eabg2661. doi: 10.1126/sciadv.abg2661. Print 2021 Aug. PubMed
Replacing the SpCas9 HNH domain by deaminases generates compact base editors with an alternative targeting scope. Villiger L, Schmidheini L, Mathis N, Rothgangl T, Marquart K, Schwank G. Mol Ther Nucleic Acids. 2021 Aug 26;26:502-510. doi: 10.1016/j.omtn.2021.08.025. eCollection 2021 Dec 3. PubMed

Associated Plasmids
Editing Properties of Base Editors with SpCas9-NG in Discarded Human Tripronuclear Zygotes. Liu X, Zhou X, Li G, Huang S, Sun W, Sun Q, Li L, Huang X, Liu J, Wang L. CRISPR J. 2021 Oct;4(5):710-727. doi: 10.1089/crispr.2021.0036. PubMed
Simultaneous high-efficiency base editing and reprogramming of patient fibroblasts. Jalil S, Keskinen T, Maldonado R, Sokka J, Trokovic R, Otonkoski T, Wartiovaara K. Stem Cell Reports. 2021 Nov 16. pii: S2213-6711(21)00552-X. doi: 10.1016/j.stemcr.2021.10.017. PubMed

Associated Plasmids
Publicly Available hiPSC Lines with Extreme Polygenic Risk Scores for Modeling Schizophrenia. Dobrindt K, Zhang H, Das D, Abdollahi S, Prorok T, Ghosh S, Weintraub S, Genovese G, Powell SK, Lund A, Akbarian S, Eggan K, McCarroll S, Duan J, Avramopoulos D, Brennand KJ. Complex Psychiatry. 2021 Feb;6(3-4):68-82. doi: 10.1159/000512716. Epub 2020 Nov 2. PubMed
A synonymous mutation in IGF-1 impacts the transcription and translation process of gene expression. Wang SY, Cheng YY, Liu SC, Xu YX, Gao Y, Wang CL, Wang ZG, Feng TQ, Lu GH, Song J, Xia PJ, Hao LL. Mol Ther Nucleic Acids. 2021 Aug 19;26:1446-1465. doi: 10.1016/j.omtn.2021.08.007. eCollection 2021 Dec 3. PubMed
High expression of uracil DNA glycosylase determines C to T substitution in human pluripotent stem cells. Park JC, Jang HK, Kim J, Han JH, Jung Y, Kim K, Bae S, Cha HJ. Mol Ther Nucleic Acids. 2021 Nov 29;27:175-183. doi: 10.1016/j.omtn.2021.11.023. eCollection 2022 Mar 8. PubMed
Highly efficient A-to-G base editing by ABE8.17 in rabbits. Zhao D, Qian Y, Li J, Li Z, Lai L. Mol Ther Nucleic Acids. 2022 Jan 28;27:1156-1163. doi: 10.1016/j.omtn.2022.01.019. eCollection 2022 Mar 8. PubMed
PPARgamma phase separates with RXRalpha at PPREs to regulate target gene expression. Li Z, Luo L, Yu W, Li P, Ou D, Liu J, Ma H, Sun Q, Liang A, Huang C, Chi T, Huang X, Zhang Y. Cell Discov. 2022 Apr 26;8(1):37. doi: 10.1038/s41421-022-00388-0. PubMed
Trapping of CDC42 C-terminal variants in the Golgi drives pyrin inflammasome hyperactivation. Nishitani-Isa M, Mukai K, Honda Y, Nihira H, Tanaka T, Shibata H, Kodama K, Hiejima E, Izawa K, Kawasaki Y, Osawa M, Katata Y, Onodera S, Watanabe T, Uchida T, Kure S, Takita J, Ohara O, Saito MK, Nishikomori R, Taguchi T, Sasahara Y, Yasumi T. J Exp Med. 2022 Jun 6;219(6). pii: 213184. doi: 10.1084/jem.20211889. Epub 2022 Apr 28. PubMed
Engineering of near-PAMless adenine base editor with enhanced editing activity and reduced off-target. Cao X, Guo J, Huang S, Yu W, Li G, An L, Li X, Tao W, Liu Q, Huang X, Jin X, Ma X. Mol Ther Nucleic Acids. 2022 May 4;28:732-742. doi: 10.1016/j.omtn.2022.04.032. eCollection 2022 Jun 14. PubMed

Associated Plasmids
Therapeutic base editing and prime editing of COL7A1 mutations in recessive dystrophic epidermolysis bullosa. Hong SA, Kim SE, Lee AY, Hwang GH, Kim JH, Iwata H, Kim SC, Bae S, Lee SE. Mol Ther. 2022 Aug 3;30(8):2664-2679. doi: 10.1016/j.ymthe.2022.06.005. Epub 2022 Jun 10. PubMed
PAM-flexible dual base editor-mediated random mutagenesis and self-activation strategies to improve CRISPRa potency. Lau CH, Huang S, Lam RHW, Tin C. Mol Ther Methods Clin Dev. 2022 May 29;26:26-37. doi: 10.1016/j.omtm.2022.05.005. eCollection 2022 Sep 8. PubMed
Generation of corrected hiPSC clones from a Cornelia de Lange Syndrome (CdLS) patient through CRISPR-Cas-based technology. Umbach A, Maule G, Kheir E, Cutarelli A, Foglia M, Guarrera L, Fava LL, Conti L, Garattini E, Terao M, Cereseto A. Stem Cell Res Ther. 2022 Sep 2;13(1):440. doi: 10.1186/s13287-022-03135-0. PubMed
Translational enhancement by base editing of the Kozak sequence rescues haploinsufficiency. Ambrosini C, Destefanis E, Kheir E, Broso F, Alessandrini F, Longhi S, Battisti N, Pesce I, Dassi E, Petris G, Cereseto A, Quattrone A. Nucleic Acids Res. 2022 Oct 14;50(18):10756-10771. doi: 10.1093/nar/gkac799. PubMed

Associated Plasmids
Mechanisms and significance of tissue-specific MICU regulation of the mitochondrial calcium uniporter complex. Tsai CW, Rodriguez MX, Van Keuren AM, Phillips CB, Shushunov HM, Lee JE, Garcia AM, Ambardekar AV, Cleveland JC Jr, Reisz JA, Proenza C, Chatfield KC, Tsai MF. Mol Cell. 2022 Oct 6;82(19):3661-3676.e8. doi: 10.1016/j.molcel.2022.09.006. PubMed
Correction of DMD in human iPSC-derived cardiomyocytes by base-editing-induced exon skipping. Wang P, Li H, Zhu M, Han RY, Guo S, Han R. Mol Ther Methods Clin Dev. 2022 Dec 2;28:40-50. doi: 10.1016/j.omtm.2022.11.010. eCollection 2023 Mar 9. PubMed
Functional restoration of a CFTR splicing mutation through RNA delivery of CRISPR adenine base editor. Amistadi S, Maule G, Ciciani M, Ensinck MM, De Keersmaecker L, Ramalho AS, Guidone D, Buccirossi M, Galietta LJV, Carlon MS, Cereseto A. Mol Ther. 2023 Jun 7;31(6):1647-1660. doi: 10.1016/j.ymthe.2023.03.004. Epub 2023 Mar 9. PubMed
Generation of a human embryonic stem cell line WAe009-A-79 carrying a long QT syndrome mutation in KCNQ1. Wang H, Guo T, Lan F. Stem Cell Res. 2023 Aug;70:103119. doi: 10.1016/j.scr.2023.103119. Epub 2023 May 10. PubMed
Precise base editing without unintended indels in human cells and mouse primary myoblasts. Yoon DE, Kim NR, Park SJ, Jeong TY, Eun B, Cho Y, Lim SY, Lee H, Seong JK, Kim K. Exp Mol Med. 2023 Dec;55(12):2586-2595. doi: 10.1038/s12276-023-01128-4. Epub 2023 Dec 1. PubMed

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