jk089_h12
(Plasmid
#112151)
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PurposeC. thermophilum DASH/Dam1 complex (construct used for cryo-EM structure determination)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 112151 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepeAA.11 and peDK.11
- Total vector size (bp) 11827
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth TemperatureRoom Temperature
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Growth Strain(s)DH5alpha
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Growth instructionsIMPORTANT: Grow at room temperature to prevent recombination!
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameC. thermophilum DASH/Dam1 complex
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Alt namepeAA.11.c_the.dash_003.peDK.11.c_the.dash_012
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Tags
/ Fusion Proteins
- his tag on Spc34 (C terminal on insert)
- strep tag on Dad1 (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Screening multiple colonies/gel purification of plasmid may be required due to recombination potential of plasmid
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
jk089_h12 was a gift from Stephen Harrison (Addgene plasmid # 112151 ; http://n2t.net/addgene:112151 ; RRID:Addgene_112151) -
For your References section:
Structure of the DASH/Dam1 complex shows its role at the yeast kinetochore-microtubule interface. Jenni S, Harrison SC. Science. 2018 May 4;360(6388):552-558. doi: 10.1126/science.aar6436. 10.1126/science.aar6436 PubMed 29724956
Map uploaded by the depositor.
