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Purpose
This lentiviral CRISPR library targets 1,594 primary human miRNA genes. The library contains 4-5 gRNAs per gene and 1,000 non-targeting control gRNAs for a total of 8,382 gRNAs.
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Vector Backbone
pLX-sgRNA-BfuAI-2k - does not express Cas9
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Depositing Labs
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | ||
|---|---|---|---|---|---|---|
| Pooled Library | 112200 | pLX-miR gRNA pooled library† | 1 | $ 430 | Add to Cart | |
† A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with pCW-Cas9 (Addgene #50661) or otherwise with cell lines already expressing Cas9.
Library Details
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SpeciesHuman
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Genes targeted1,594
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gRNAs8,382
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Controls1,000 non-targeting
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Lentiviral Generation3rd
Library Shipping
This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.
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Volume∼20µL
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Concentration50ng/µL
Resource Information
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Protocols
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Depositor Data
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Terms and Licenses
Depositor Comments
Depositor recommends publicly available algorithms such as btrim and bowtie for data analysis.
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLX-miR library was a gift from Ren-Jang Lin (Addgene #112200) -
For your References section:
MicroRNA-focused CRISPR-Cas9 Library Screen Reveals Fitness-Associated miRNAs. Kurata JS, Lin RJ. RNA. 2018 May 2. pii: rna.066282.118. doi: 10.1261/rna.066282.118. PubMed 29720387