Addgene - hCathepsin L Plasmid Data

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11250: hCathepsin L
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11185: 1574 pSG5L F…
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11177: 1571 pcDNA3 …

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Plasmid 11250: hCathepsin L

Gene/insert name:
cathepsin L
Species:
H. sapiens (human)
Entrez Gene:
CTSL1 (MEP, CATL, CTSL, FLJ31037)
Fusion protein or tag:
C9 (GTETSQVAPA)
Terminal:
C terminal on insert
Vector backbone:
pcDNA3.1(-)
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert:
5428
Cloning site 5':
XbaI
Site destroyed during cloning:
No
Cloning site 3':
PmeI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Hyeryun Choe
Terms and Licenses:
MTA

Comments:

See author's map for more information.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: SARS coronavirus, but not human coronavirus NL63, utilizes cathepsin L to infect ACE2-expressing cells. Huang et al (J Biol Chem. 2005 Dec 8. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11250" in your Materials and Methods section.