Lentiviral-S-tdTomato
(Plasmid #112579)

• Purpose: Expressing Shuttle-tdTomato, or NLS-NES-tagged tdTomato in a Lentiviral vector
• Depositing Lab: Jeffrey Rothstein
• Publication: Zhang et al Nature. 2015 Sep 3;525(7567):56-61. doi: 10.1038/nature14973. Epub 2015 Aug 26.

Backbone

• Vector backbone: pLenti-C-Myc-DDK
• Backbone manufacturer: Origene
• Backbone size w/o insert (bp): 6400
• Total vector size (bp): 7800
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NLS-tdTomato-NES
• Alt name: Shuttle-tdTomato, S-tdTomato
• Species: Fluorescent protein tdTomato
• Insert Size (bp): 1300
• Tags / Fusion Proteins:
  • nuclear localization sequence (N terminal on insert)
  • nuclear export sequence (C terminal on insert)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: From Svetlana Vidensky, a technician in the Rothstein lab.
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry