pCR-Blunt_hNANOG
(Plasmid
#112639)
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PurposePlasmid for PCR amplification of hNANOG DNA template for in vitro transcription
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 112639 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCR-Blunt
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Backbone manufacturerThermoFisher
- Backbone size w/o insert (bp) 3500
- Total vector size (bp) 4550
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Vector typePCR Template
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehNANOG
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SpeciesH. sapiens (human)
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Insert Size (bp)1086
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Entrez GeneNANOG
- Promoter T7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer M13 Fw
- 3′ sequencing primer M13 Rv (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCR-Blunt_hNANOG was a gift from Ganna Bilousova (Addgene plasmid # 112639 ; http://n2t.net/addgene:112639 ; RRID:Addgene_112639) -
For your References section:
RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells. McGrath PS, Diette N, Kogut I, Bilousova G. J Vis Exp. 2018 Nov 26;(141). doi: 10.3791/58687. 10.3791/58687 PubMed 30531717
Map uploaded by the depositor.
