pEBLindv2
(Plasmid
#113108)
-
PurposeVector encoding the EL222 gene under constitutive promoter J23106.
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 113108 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepBbE8K
-
Modifications to backboneFirst insert: J23106-BBa_B0034-EL222-Bba_B0015; followed by second insert: BindF1-Bba_B0034-RFP-Bba_B0015
-
Vector typeBacterial Expression ; Cell-free
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameEL222
-
SpeciesE. coli
- Promoter J23106
-
Tag
/ Fusion Protein
- RFP (C terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pEBLindv2 was a gift from Chueh Loo Poh (Addgene plasmid # 113108) -
For your References section:
Cell-Free Optogenetic Gene Expression System. Jayaraman P, Yeoh JW, Jayaraman S, Teh AY, Zhang J, Poh CL. ACS Synth Biol. 2018 Apr 20;7(4):986-994. doi: 10.1021/acssynbio.7b00422. Epub 2018 Apr 4. 10.1021/acssynbio.7b00422 PubMed 29596741
Map uploaded by the depositor.
