pKJ-1
(Plasmid
#11333)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 11333 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepUC18
- Backbone size w/o insert (bp) 2700
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Vector typeBacterial Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepgk-1 5' and 3' regions
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Alt namephosphoglycerate kinase-1
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Alt namePGKneo
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SpeciesM. musculus (mouse)
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Insert Size (bp)1800
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Entrez GenePgk1 (a.k.a. Pgk-, Pgk-1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Includes 500 bp mouse PGK promoter, 832 bp neo, 460 bp PGK 3' end.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKJ-1 was a gift from Michael McBurney (Addgene plasmid # 11333 ; http://n2t.net/addgene:11333 ; RRID:Addgene_11333) -
For your References section:
Cloning and expression of the mouse pgk-1 gene and the nucleotide sequence of its promoter. Adra CN, Boer PH, McBurney MW. Gene. 1987 . 60(1):65-74. 10.1016/0378-1119(87)90214-9 PubMed 3440520
Map uploaded by the depositor.
