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pBABE-hygro NM23 H1 Pro
(Plasmid #11366)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11366 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBABE-hygro
  • Backbone size w/o insert (bp) 5558
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NM23 H1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    500
  • Mutation
    Pro96 mutation to Ser (reduces GAP activity).
  • Entrez Gene
    NME1 (a.k.a. AWD, GAAD, NB, NBS, NDKA, NDPK-A, NDPKA, NM23, NM23-H1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer pBABE 5'
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE-hygro NM23 H1 Pro was a gift from Ronald Kahn (Addgene plasmid # 11366 ; http://n2t.net/addgene:11366 ; RRID:Addgene_11366)
  • For your References section:

    Interaction of the Ras-related protein associated with diabetes rad and the putative tumor metastasis suppressor NM23 provides a novel mechanism of GTPase regulation. Zhu J, Tseng YH, Kantor JD, Rhodes CJ, Zetter BR, Moyers JS, Kahn CR. Proc Natl Acad Sci U S A. 1999 Dec 21. 96(26):14911-8. 10.1073/pnas.96.26.14911 PubMed 10611312