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Purpose(Empty Backbone) Gateway destination vector for lentiviral expression
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 113668 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepLX_TRC311
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Backbone manufacturerGenetic Perturbation Platform
- Backbone size (bp) 9879
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Vector typeMammalian Expression, Lentiviral
- Promoter EF1a
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)ccdB Survival
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Growth instructionsdepositor recommends Carbenicillin (100ug/ml) and chloramphenicol (50ug/ml) for bacterial resistance.
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Copy numberHigh Copy
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLX_TRC311 was a gift from John Doench (Addgene plasmid # 113668 ; http://n2t.net/addgene:113668 ; RRID:Addgene_113668)
