pDuEx2-NIR-GECO1
(Plasmid #113680)

• Purpose: Bacterial and mammalian expression plasmid for near-infrared fluorescent Ca2+ indicator NIR-GECO1
• Depositing Lab: Robert Campbell
• Publication: Qian et al Nat Methods. 2019 Feb;16(2):171-174. doi: 10.1038/s41592-018-0294-6. Epub 2019 Jan 21.

Backbone

• Vector backbone: pcDNA
• Backbone size w/o insert (bp): 6160
• Total vector size (bp): 7648
• Vector type: Mammalian Expression, Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NIR-GECO1
• Species: Synthetic
• Insert Size (bp): 1488
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: CCA TGG AGT GGG ATT TTT TC

Resource Information

• Supplemental Documents:
  • pcDuEx2-NIRGECO1.gb
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry