pGEM-gate
(Plasmid #113758)

• Purpose: Gateway destination vector only containing the Gateway cassette and pGEM-T Easy backbone. This can be used with Gateway-compatible Homology-Directed Repair.
• Depositing Lab: Magdalena Bezanilla
• Publication: Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. doi: 10.1002/pld3.168. eCollection 2019 Sep.

Backbone

• Vector backbone: pGEM-T easy
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3048
• Vector type: Gateway Destination Vector

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Growth instructions: May use DB3.1 cells instead of ccdB Survival
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: gateway cassette
• Insert Size (bp): 1703

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: M13 Forward
• 3′ sequencing primer: M13 Reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Use this vector for 3-way multi-site Gateway reactions with two regions of homology flanking an insert - this final vector can be used as a template for repair (homology-directed repair) after CRISPR double-strand break. This plasmid was constructed by A-tailing the Gateway cassette (R1R2) and blunt/TA Ligase reaction with pGEM-T Easy to create pGEM-gate.