pJLI-SUP35 (delta)2-5
(Plasmid #1138)

• Depositing Lab: Susan Lindquist
• Publication: Liu et al Nature 1999 Aug 5;400(6744):573-6.

Backbone

• Vector backbone: pRS306
• Backbone size w/o insert (bp): 4299
• Vector type: Yeast Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: sup35 delta 2-5 mutant, 5' and 3' flanking regions
• Alt name: sup35
• Alt name: sup35 upstream and downstream
• Alt name: NM domain
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 4115
• Mutation: last four repeats of sup35 N domain deleted, but rest of ORF intact. Also includes sup35 5' and 3' flanking regions
• GenBank ID: AY553990
• Entrez Gene: SUP35 (a.k.a. YDR172W, GST1, PNM2, SAL3, SUF12, SUP2, SUP36)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI-refer to strategy in manuscript (not destroyed)
• 3′ cloning site: SacII- refer to strategy in manuscript (not destroyed)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry