Addgene - mPrP-pDN Plasmid Data

Plasmid 1141: mPrP-pDN

Gene/insert name:
PrP
Alt name:
mouse prion protein
Species:
M. musculus (mouse)
GenBank ID:
NT_039207
Entrez Gene:
Prnp (RP23-401J24.1, AA960666, AI325101, CD230, PrP, PrP<C>, PrPC, PrPSc, Prn-i, Prn-p, Sinc, prP27-30, prP33-35C)
Mutation:
mPrP(23-231) fused with KAR2 signal seqence under the control of Gal promoter in a CEN plasmid (replacing the GFP in pDN182, #6637). Insert can be cleaved out by ClaI and XbaI. Two extra amino acids, I, D, included in the juction between PrP and Kar2 signal seqence. NO SEQUENCE PROVIDED BY CREATOR.
Vector backbone:
pDN182
(Search Vector Database)
Vector type:
Yeast Expression
Cloning site 5':
Cla1
Site destroyed during cloning:
No
Cloning site 3':
Xba1
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
3' sequencing primer:
n/a
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Person or lab that originally
cloned the gene/insert:
unknown
Sequence: View sequences (2)
Principal Investigator:
Susan Lindquist
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1141" in your Materials and Methods section.