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mPrP-pDN
(Plasmid #1141)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 1141 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDN182
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PrP
  • Alt name
    mouse prion protein
  • Species
    M. musculus (mouse)
  • Mutation
    mPrP(23-231) fused with KAR2 signal seqence under the control of Gal promoter in a CEN plasmid (replacing the GFP in pDN182, #6637). Insert can be cleaved out by ClaI and XbaI. Two extra amino acids, I, D, included in the juction between PrP and Kar2 signal seqence. NO SEQUENCE PROVIDED BY CREATOR.
  • GenBank ID
    NT_039207
  • Entrez Gene
    Prnp (a.k.a. CD230, PrP, PrP<C>, PrPC, PrPSc, Prn-i, Prn-p, Sinc, prP27-30, prP33-35C)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Cla1 (not destroyed)
  • 3′ cloning site Xba1 (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mPrP-pDN was a gift from Susan Lindquist (Addgene plasmid # 1141)
  • For your References section:

    De novo generation of a PrPSc-like conformation in living cells. Ma J, Lindquist S. Nat Cell Biol 1999 Oct;1(6):358-61. 10.1038/14053 PubMed 10559963