mPrP-pDN
(Plasmid
#1141)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 1141 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepDN182
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePrP
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Alt namemouse prion protein
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SpeciesM. musculus (mouse)
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MutationmPrP(23-231) fused with KAR2 signal seqence under the control of Gal promoter in a CEN plasmid (replacing the GFP in pDN182, #6637). Insert can be cleaved out by ClaI and XbaI. Two extra amino acids, I, D, included in the juction between PrP and Kar2 signal seqence. NO SEQUENCE PROVIDED BY CREATOR.
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GenBank IDNT_039207
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Entrez GenePrnp (a.k.a. CD230, PrP, PrP<C>, PrPC, PrPSc, Prn-i, Prn-p, Sinc, prP27-30, prP33-35C)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Cla1 (not destroyed)
- 3′ cloning site Xba1 (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byunknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mPrP-pDN was a gift from Susan Lindquist (Addgene plasmid # 1141) -
For your References section:
De novo generation of a PrPSc-like conformation in living cells. Ma J, Lindquist S. Nat Cell Biol 1999 Oct;1(6):358-61. 10.1038/14053 PubMed 10559963