pAAV-hSyn-sbGLuc-B7-EYFP
(Plasmid
#114110)
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Purposefusion protein of Gaussia luciferase variant slow burn, B7 transmembrane region, and enhanced yellow fluorescent protein; control for bioluminescent optogenetics
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 114110 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepAAV
- Backbone size w/o insert (bp) 4666
- Total vector size (bp) 6211
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert 1
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Gene/Insert nameGaussia luciferase, slow burn
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Alt namesbGLuc
Gene/Insert 2
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Gene/Insert nameB7 hinge region
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Alt nameB7
Gene/Insert 3
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Gene/Insert nameEnhanced Yellow Fluorescent Protein
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Alt nameEYFP
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-hSyn-sbGLuc-B7-EYFP was a gift from Ute Hochgeschwender (Addgene plasmid # 114110 ; http://n2t.net/addgene:114110 ; RRID:Addgene_114110) -
For your References section:
The BioLuminescent-OptoGenetic in vivo response to coelenterazine is proportional, sensitive, and specific in neocortex. Gomez-Ramirez M, More AI, Friedman NG, Hochgeschwender U, Moore CI. J Neurosci Res. 2020 Mar;98(3):471-480. doi: 10.1002/jnr.24498. Epub 2019 Sep 23. 10.1002/jnr.24498 PubMed 31544973