M3-PAmcherry1
(Plasmid #114189)

• Purpose: studying clustering of M3 receptors by superresolution microscopy (PALM)
• Depositing Lab: Aleksandra Radenovic
• Publication: Radenovic Lab plasmids (unpublished)

Backbone

• Vector backbone: pPA-mCherry N1
• Backbone size w/o insert (bp): 4727
• Modifications to backbone: pcDNA3.1-M3 receptor (wt) was purchased from cDNA.org. They amplified M3 by PCR from human whole brain cDNA (Clontech).
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Muscarin acetylcholine receptor 3
• Alt name: M3
• Alt name: CHRM3
• Species: H. sapiens (human)
• Insert Size (bp): 1790
• Entrez Gene: CHRM3 (a.k.a. EGBRS, HM3, PBS, m3AChR)
• Promoter: SV40
• Tags / Fusion Proteins:
  • 3xHA (N terminal on insert)
  • PAmCherry (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: ApaI (not destroyed)
• 5′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry