|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||114195||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonelentiCRISPR v2
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Growth Strain(s)NEB Stable
Insert Size (bp)5865
- Promoter EF1a
/ Fusion Protein
- FLAG (N terminal on insert)
- Cloning method Gibson Cloning
- 5′ sequencing primer GTTTATTACAGGGACAGCAGA (Common Sequencing Primers)
Terms and Licenses
Addgene #63798 into lentivirus backbone and addition of N-term FLAG tag and EF1a promoter . Please visit https://www.biorxiv.org/content/early/2018/07/17/371500 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:lenti EF1a-FLAG-dCas9-VPR was a gift from Jeremy Day (Addgene plasmid # 114195 ; http://n2t.net/addgene:114195 ; RRID:Addgene_114195)
For your References section:A neuron-optimized CRISPR/dCas9 activation system for robust and specific gene regulation. Savell K, Bach S, Zipperly M, Revanna J, Goska N, Tuscher J, Duke C, Sultan F, Burke J, Williams D, Ianov L, Day J. bioRxiv 371500 /10.1101/371500