mPrP(3F4)-Cyc-P2UGPD
(Plasmid
#1143)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 1143 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepGAL
- Backbone size w/o insert (bp) 5586
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHsp104 (361-548)
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Alt nameHsp104
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)583
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Mutationaa361-548 of Hsp104
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Entrez GeneHSP104 (a.k.a. YLL026W)
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Tag
/ Fusion Protein
- 6X His + TEV cleavage (N terminal on backbone)
Resource Information
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A portion of this plasmid was derived from a plasmid made byunknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mPrP(3F4)-Cyc-P2UGPD was a gift from Susan Lindquist (Addgene plasmid # 1143) -
For your References section:
De novo generation of a PrPSc-like conformation in living cells. Ma J, Lindquist S. Nat Cell Biol 1999 Oct;1(6):358-61. 10.1038/14053 PubMed 10559963