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mPrP(3F4)-Cyc-P2UGPD
(Plasmid #1143)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1143 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pGAL
  • Backbone size w/o insert (bp) 5586
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Hsp104 (361-548)
  • Alt name
    Hsp104
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    583
  • Mutation
    aa361-548 of Hsp104
  • Entrez Gene
    HSP104 (a.k.a. YLL026W)
  • Tag / Fusion Protein
    • 6X His + TEV cleavage (N terminal on backbone)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mPrP(3F4)-Cyc-P2UGPD was a gift from Susan Lindquist (Addgene plasmid # 1143)

  • For your References section:

    De novo generation of a PrPSc-like conformation in living cells. Ma J, Lindquist S. Nat Cell Biol 1999 Oct;1(6):358-61. 10.1038/14053 PubMed 10559963
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